Results
| PMID | 9591504 |
| Gene Name | IL8 |
| Condition | Endometriosis |
| Association |
Associated |
| Population size | 40 |
| Population details | 40 women who underwent either laparoscopy or laparoscopic surgery |
| Sex | Female |
| Infertility type | Female infertility |
| Associated genes | IL-8 |
| Other associated phenotypes |
Endometriosis |
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Fertil Steril. 1998 May;69(5):924-30. Iwabe, T| Harada, T| Tsudo, T| Tanikawa, M| Onohara, Y| Terakawa, N Department of Obstetrics and Gynecology, Tottori University School of Medicine, Yonago, Japan. OBJECTIVE: To investigate the role of interleukin-8 (IL-8) in peritoneal fluid of patients with endometriosis in the pathogenesis of endometriosis. DESIGN: Peritoneal fluid was collected by laparoscopy. Endometrial and endometriotic stromal cells were obtained from normal endometrium and from chocolate cyst linings of the ovary. SETTING: Department of Obstetrics and Gynecology of Tottori University Hospital, Yonago, Japan. PATIENT(S): Forty women who underwent either laparoscopy or laparoscopic surgery. MAIN OUTCOME MEASURE(S): The peritoneal fluid concentration of IL-8 was analyzed by enzyme-linked immunosorbent assay, and the correlation between the IL-8 concentration and the extent of active endometriosis was investigated. The effect of IL-8 on cell proliferation was examined by tetrazolium bromide and thymidine incorporation. The expression of IL-8 receptor was examined in stromal cells by reverse transcription polymerase chain reaction. RESULT(S): The level of IL-8 in peritoneal fluid was significantly higher in patients with endometriosis than in patients without endometriosis. A significant correlation was noted with the extent of active endometriosis. Interleukin-8 significantly increased the number of cells and DNA synthesis in the endometrial and endometriotic stromal cells in a dose-dependent manner. Transcripts of IL-8 receptor type A were detected in stromal cells. CONCLUSION(S): The present study suggests that IL-8 found in the peritoneal fluid of patients with endometriosis contributes to the pathogenesis of endometriosis. Mesh Terms: Antigens, CD/analysis| Ascitic Fluid/*chemistry| Cell Division| Cells, Cultured| Endometriosis/etiology/*metabolism/pathology| Female| Humans| Interleukin-8/*analysis| Receptors, Interleukin/analysis| Receptors, Interleukin-8A| Stromal Cells/phy |
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